AnaChips™ for Biophysics Applications

Biomolecular interactions are studied on AnaLight® instruments by injecting one or more of the interacting partners into the flow over the prepared surface of an AnaChip™, on which the other interacting biomolecule has been immobilised. Biomolecular immobilisation onto the AnaChip™ surface can be undertaken by either covalent linker, biospecific surface chemistry or charge (physisorption).

Farfield offers a range of AnaChips™ suitable for biophysical applications. The most suitable AnaChip™ surface for any biomolecular immobilisation should be assessed according to the properties of the molecule to be immobilised and the objectives behind the measurements being taken on the AnaLight®.

Further technical information on biophysical immobilisation strategies can be found in the InfoZone.

Surface: Unmodified silicon oxynitride giving hydroxyl surface chemistry carrying negative charge. Positively charged molecules can be immobilised to the surface by physisorption. Hydroxyl surface chemistry is also available for customer-defined covalent immobilisations using silane chemistry with or without linkers.

Use: Suitable for study of proteins from low to high molecular weight, peptides, carbohydrates, lipids, and liposomes.

Ordering information: Part No. 2007-110c (pack of 5)

Unmodified AnaChip™ (as above) additionally supplied with Manufacturers Test Certificate guaranteeing specifications and traceability. For use in AnaLight® Qualification Procedures and validated applications, where traceability to national and international standards is required.

Ordering information: Part No. 2007-162c (pack of 1)

Surface: Amine functionalised surface. The first choice for covalent immobilisation of biomolecules. Couple to amine groups on the AnaChip™ surface via -NH2, -CHO or -SH groups on target biomolecule, using one of a range of linker chemistries. Amine surface also carries positive charge, allowing the immobilisation of negatively charged biomolecules by physisorption.

Use: Suitable for study of proteins from low to high molecular weight, peptides, nucleic acids, carbohydrates, lipids, and liposomes.

Ordering information: Part No. 2007-111c (pack of 5)

Surface: Thiol functionalised surface for covalent immobilisation of biomolecules. Couple to thiol groups on the AnaChip™ surface via -NH2 or -SH groups on target biomolecule, with or without linker chemistry. Thiol surface also suitable for the physisorption of more hydrophobic molecules.

Use: Suitable for study of DNA, RNA, proteins from low to high molecular weight, peptides

Ordering information: Part No. 2007-113c (pack of 5)

Surface: C18 hydrocarbon functionalised surface for adsorption of hydrophobic biomolecules, usually those associated with membrane environments. Can also be used for the adsorption of lipids to form hemibilayers as membrane mimics.

Use: Suitable for adsorption of hydrophobic proteins and peptides and forming lipid-based mimics for membrane protein studies.

Ordering information: 2007-112c (pack of 5)

Farfield also supplies a range of AnaKit™ products providing reagents and protocols to expand the functionality and applications of the AnaChip™ range. Contact sales@farfield-group.com for details.